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Image Search Results
Journal: Journal of Clinical Medicine
Article Title: C-Reactive Protein (CRP) Blocks the Desensitization of Agonistic Stimulated G Protein Coupled Receptors (GPCRs) in Neonatal Rat Cardiomyocytes
doi: 10.3390/jcm11041058
Figure Lengend Snippet: CRP blocks the desensitization of stimulated neonatal rat cardiomyocytes with ISO. Difference of cardiomyocyte beating rates [Δ Beating rate/15 s] modulated by adrenoceptor agonist isoprenaline (ISO) with or without CRP co-incubation. ( A ) Mean of 3–7 independent experiments ± standard deviation is depicted. Arrows indicate stimulation with respective agonists. Grey area indicates washing step. After the washing step, the agonist was added again but no CRP. To test if CRP itself influences the beating rate, cells were incubated with CRP and no change could be observed after 5 min (grey circle). Significance of difference between CRP incubated and control group and mAb group at 120 min and 130 min was calculated with two-way ANOVA with Bonferroni post-hoc test. ( B ) Same experimental setup as depicted in ( A ), but only the beating rate at the last time point (130 min) is shown as mean of 3–5 independent experiments ± standard deviation or as single value (20–2 µg/mL CRP). Significance of difference between 40 µg/mL CRP and control (only ISO) was calculated with student’s t -test. ** p < 0.01. *** p < 0.001. ns: not significant.
Article Snippet: The
Techniques: Incubation, Standard Deviation, Control